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| Cooperative Verification of the Value of GM Soybean Mon89788 Matrix Reference Materials |
| ZHAG Ling1,SHENG Ling-hui1,WANG Jing1,CAO Ying-long2,LIU Fang-fang3,SUI Zhi-wei1,LU Chang-ming2 |
1.National Institute of Metrology, Beijing 100013, China
2.Oil Crops Research Institute of Chinese Academy of Agricultural Sciences, Wuhan, Hubei 430062, China
3.Beijing University of Chemical Technology, Beijing 100029, China |
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Abstract Three levels of genetically modified soybeans Mon89788 matrix reference material are developed. Genetically modified soybean genomic DNA extraction and quantitative PCR of transgenic components methods are researched. A multi-laboratory verification and uncertainty evaluation are carried out too.The analysis of multi-laboratory value verification shows that in three different levels of genetically modified soybean Mon89788 reference materials, the transgenic gene / endogenous genes reference value and uncertainty is 0.35%±0.17%,0.61%±0.14%,3.17%±1.70% (k=2), respectively.This set of reference materials that the matrix is soybean grand can be used for the quantitative detection of genetically modified soybeans content.
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［1］ISO Guide 31-2000,Reference materials-contents of certificates and labels［S］.
［2］Drries H, Remus I, Grnewald A,et al.Development of a qualitative, multiplex real-time PCR kit for screening of genetically modified organisms (GMOs) ［J］.Analytical and Bioanalytical Chemistry,2009, 396(6), 2043-2054.
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［5］李飞武,李葱葱,董立明,等.转基因大豆MON89788转化体特异性定性PCR检测［J］.安徽农业科学,2010, 38(13):82-86.
［6］全浩,韩永志．标准物质及其应用技术［M］．第二版．北京:中国标准出版社,2003． |
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2011, Vol. 32 
