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| Study on Freeze-thaw Cycles on Taqman MGB Stability |
| ZANG Chao1,WANG Jing1,LI Liang1,SUI Zhi-wei2,YU Xiao-bo2,LI Peng1 |
1. National Institute of Metrology, Beijing 100013, China
2. China Jiliang University, Hangzhou, Zhejiang 310018, China |
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Abstract The stability of Taqman MGB probes is crucial for the accuracy of qPCR quantification. However, the stability is easy to be affected by multi freeze thawing process of transportations, storage surroundings and usage conditions. The freeze thawing stability of MGB probe is quantified measured by HPLC and the influence to qPCR is evaluated with multi freeze thawing probes. The results show that a 30-time freeze thawing process is tolerable for MGB probes. QPCR quantitation accuracy decreases together with probe concentration. Therefore, reducing freeze thawing process of MGB probes is offered to keep the stability of MGB probes when experiments.
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2011, Vol. 32 
