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| Cooperative Value Verification and Uncertainty Evaluation for GM Maize TC1507 Matrix Reference Materials |
| ZANG Chao1,FU Bo-qiang1,SONG Gui-wen2,MENG Ya-nan3,WANG Jing1,DONG Lian-hua1,YU Xiao-bo1,2,SUI Zhi-wei1,LI Liang1,YANG Li-tao3 |
1. National Institute of Metrology, Beijing 100013, China
2. Science and Technology Development Center, The Ministry of Agriculture, Beijing 100122, China
3. School of Life Science and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China
4. College of Life Science, China Jiliang University, Hangzhou, Zhejiang 310018, China |
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Abstract Seven laboratories are organized to verify the value of three levels genetically modified TC1507 maize matrix reference material.The uncertainties of the multi-laboratories values are evaluated. The cooperative Rt-PCR results show that the values and uncertainties of genetically modified TC1507 maize matrix reference material are 0.41%±0.22%, 1.1%±0.4%, 4.5%±1.2% (k=2), respectively.
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［1］陈元生,徐小云.玉米重大害虫亚洲玉米螟综合治理策略［J］.广东农业科学,2011, 38(2):80-83.
［2］Drries H, Remus I, Grnewald A, et al. Development of a qualitative, multiplex real-time PCR kit for screening of genetically modified organisms (GMOs)［J］. Analytical and Bioanalytical Chemistry, 2009, 396(6), 2043-2054.
［3］Mazzara M, Foti N, Price S, et al. Event-specific method for the quantitation of maize line TC1507 using real-time PCR-validation report and protocol -sampling and DNA extraction of maize TC15O7 ［R］.http://gmo-crl.jrc.ec.europa.eu/gmomethods/,2011-11-10.
［4］Paz L J, Garca-Muniz N, Nadal A, et al. Interlaboratory transfer of a real-time polymeras chain reaction assay for quantitative detection of genetically modified maiz event TC-1507［J］.Journal of AOAC International, 2006, 89(5): 1347-1352.
［5］European Union Network for GMO Laboratories.Definition of minimum performance requirements for analytical methods of GMO testing ［R］.http://gmo-crl.jrc.ec.europa.eu/guidancedocs.htm,2011-11-10.
［6］全浩,韩永志.标准物质及其应用技术［M］.第2版.北京:中国标准出版社,2003. |
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2011, Vol. 32 
