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| General Principle for Uncertainty Evaluation of Enzyme Activity Measurement |
| WU Li-qing,YANG Bin,WANG Jing |
| National Institute of Metrology, Beijing 100013, China |
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Abstract The general principle for uncertainty evaluation of enzyme activity measurement is presented based on previous experiment.Totally five steps toward the uncertainty evaluation of enzyme activity measurement are introduced, including the principle of experimental condition selection and optimization, uncertainty source analysis, uncertainty component and combination graph drawing, sensitivity factor and uncertainty component calculation, standard combination uncertainty and expanded uncertainty calculation.The principle presented will lay a foundation for the accuracy and validity in the enzyme activity measurement and enzyme activity certified reference material production.
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［1］JJF 1059—1999,测量不确定度评定与表示［S］.
［2］倪育才.实用测量不确定度评定［M］.北京:中国计量出版社,2004.
［3］武利庆,谢宝民,王晶.α-淀粉酶活性测定方法研究及测量结果不确定度评估［J］.计量学报,2007,28(5A):250-255. |
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2011, Vol. 32 
