高通量测序标准物质的研究进展

doi:10.3969/j.issn.1000-1158.2024.01.18

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Abstract With the advent of next-generation sequencing technology, gene sequence has been widely studied.Due to the complexity of genetic material, technical errors introduced during sample preparation, sequencing, and analysis, and systematic biases between different sequencing platforms, high-throughput sequencing results suffer in terms of accuracy and consistency across platforms. However, the use of standard materials in the sequencing process can solve these problems. Next-generation sequencing reference materials are usually genetic materials with good characteristics or synthetic reference materials added and simulated electronic data sets. The application of next-generation sequencing reference materials will help calibrate the measurement results of next-generation sequencing and evaluate the instrument performance, which is crucial to ensure the accuracy and consistency of sequencing results.

Key words： biometrics next-generation sequencing reference material quality control

Received: 02 March 2023 Published: 22 January 2024

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TB99

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	Articles by authors
	LIU Yang
	SU Zhaozhong
	ZENG Fanjun
	YUAN Huijun
	ZHANG Yongzhuo

Cite this article:

LIU Yang,SU Zhaozhong,ZENG Fanjun, et al. Research Progress of Next-Generation Sequencing Reference Materials[J]. Acta Metrologica Sinica, 2024, 45(1): 128-134.

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http://jlxb.china-csm.org:81/Jwk_jlxb/EN/10.3969/j.issn.1000-1158.2024.01.18 OR http://jlxb.china-csm.org:81/Jwk_jlxb/EN/Y2024/V45/I1/128

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