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| Development of Quantum Dot-Doped Fluorescent Gel Reference Strip and Its Application in Calibration of Fluorescence Immunoassay Analyzer |
| TAO Qing1,WANG Le-le1,PENG Tao2,LI Lan-ying1,LIU Gang1,WEN Yan-li1 |
1. Key Laboratory of Bioanalysis and Metrology for State Market Regulation, Shanghai Institute of Measurement and Testing Technology, Shanghai 201203, China
2. National Institute of Metrology, Beijing 100029, China |
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Abstract Quantum dot-doped fluorescent gel reference strips were developed based on microfluidics and quantum dot technology. These strips were formed by uniformly mixed quantum dots and PDMS, which were pressed into capillary tubes to solidify. By controlling the concentration of quantum dots, fluorescent gel reference strips with three fluorescence intensities of high, medium, and low were prepared. The photobleaching, homogeneity, and stability of the reference strips were investigated, and the uncertainties were evaluated. The results showed that the fluorescent gel reference strips possessed good photobleaching resistance and uniformity, and can be stored stably for 180 days at room temperature in the dark. Calibration experiments on a number of fluorescent immunoassay analyzers proved that the fluorescent gel reference strips had good applicability and provided a reliable reference for the calibration of fluorescent immunoassay analyzers.
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Received: 08 May 2023
Published: 27 December 2023
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2023, Vol. 44 
