Acta Metrologica Sinica  2023, Vol. 44 Issue (10): 1612-1616    DOI: 10.3969/j.issn.1000-1158.2023.10.18
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Establishment of Measurement and Verification Method for Sequential Evaluation of Anoikis Effect on Hela Cell
XUE Zhi-chao,ZENG Jia-ming,GONG Xiao-yun,XUE Qian,DAI Xin-hua,ZHAO YangXUE Zhi-chao,ZENG Jia-ming,GONG Xiao-yun,XUE Qian,DAI Xin-hua,ZHAO Yang
Technology Innovation Center of Mass Spectrometry for State Market Regulation, Center for Advanced Measurement Science, National Institute of Metrology, Beijing 100029, China
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Abstract  Anoikis effect is a special program death for attachment cells in the body, which plays an important role in body development, tissue homeostasis, disease occurrence, and tumor metastasis. Attachment epithelial cell line Hela was hired as the experiment model to investigate the anoikis effect in detail. Living Hela cells were counted after trypsinization and floating for 5min, 30min, 1h, 2h, 4h, and 8h respectively. The activity of these cells was detected after seeding back for 24h. The results showed that floating time would severely decrease cell counting and activity of HeLa, and the floating time of attachment cell needs to be controlled during cell experiments.
Key wordsmetrology      life science metrology      cell number      cell activity      cell measurement      anoikis effect      Hela cell      floating time     
Received: 11 January 2022      Published: 10 October 2023
PACS:  TB99  
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XUE Zhi-chao
ZENG Jia-ming
GONG Xiao-yun
XUE Qian
DAI Xin-hua
ZHAO Yang
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XUE Zhi-chao,ZENG Jia-ming,GONG Xiao-yun, et al. Establishment of Measurement and Verification Method for Sequential Evaluation of Anoikis Effect on Hela Cell[J]. Acta Metrologica Sinica, 2023, 44(10): 1612-1616.
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http://jlxb.china-csm.org:81/Jwk_jlxb/EN/10.3969/j.issn.1000-1158.2023.10.18     OR     http://jlxb.china-csm.org:81/Jwk_jlxb/EN/Y2023/V44/I10/1612
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