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| Establishment of Measurement and Verification Method for Sequential Evaluation of Anoikis Effect on Hela Cell |
| XUE Zhi-chao,ZENG Jia-ming,GONG Xiao-yun,XUE Qian,DAI Xin-hua,ZHAO YangXUE Zhi-chao,ZENG Jia-ming,GONG Xiao-yun,XUE Qian,DAI Xin-hua,ZHAO Yang |
| Technology Innovation Center of Mass Spectrometry for State Market Regulation, Center for Advanced Measurement Science, National Institute of Metrology, Beijing 100029, China |
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Abstract Anoikis effect is a special program death for attachment cells in the body, which plays an important role in body development, tissue homeostasis, disease occurrence, and tumor metastasis. Attachment epithelial cell line Hela was hired as the experiment model to investigate the anoikis effect in detail. Living Hela cells were counted after trypsinization and floating for 5min, 30min, 1h, 2h, 4h, and 8h respectively. The activity of these cells was detected after seeding back for 24h. The results showed that floating time would severely decrease cell counting and activity of HeLa, and the floating time of attachment cell needs to be controlled during cell experiments.
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Received: 11 January 2022
Published: 10 October 2023
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2023, Vol. 44 
