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| Preparation of Atypical Salmonella Reference Material in Chicken and the Application in the Proficiency Testing |
| XU Qian1,2,LIU Si-yuan2,GAO Ying2,WANG Zi-quan2,SUI Zhi-wei2,CHANG Hai-yan1 |
1. College of Life Sciences, Hunan Normal University, Changsha, Hunan 410081, China
2. National Institute of Metrology, Beijing 100029, China |
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Abstract In order to solve the problem of the lack of reference material and the development level of Salmonella, atypical Salmonella NIM JZ048 was selected as the target bacteria, and Enterobacter aerogenes CICC 10293 was selected as the interference bacteria to develop atypical Salmonella reference materials in chicken.The results indicated that the quantitative standard value of Salmonella for the reference material is (1.8±0.4)×103CFU/g, the quantitative standard value of total bacterial count is (5.5±0.9)×103CFU/g, the qualitative standard value is Salmonella positive. The reference material has good uniformity and stability, and can be stably stored at -20℃ for six months.In addition, the reference material was used as the test sample for proficiency test of Salmonella qualitative detection participated by twenty-one food inspection agencies. The results of 15 agencies were qualified. Six agencies failed to detect atypical Salmonella, resulting in unqualified results. The atypical Salmonella reference material in chicken can be effectively used for the evaluation of Salmonella detection capability in food inspection agencies.
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Received: 03 August 2021
Published: 17 July 2023
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|
|
|
|
| ［2］ |
Abebe E, Gugsa G, Ahmed M. Review on major food-borne zoonotic bacterial pathogens ［J］. J Trop Med, 2020: 4674235.
|
| ［5］ |
Bumann, D, Schothorst J. Intracellular Salmonella metabolism ［J］. Cell Microbiol, 2017, 19(10),doi: 10.1111/cmi.12766.
|
| ［7］ |
Cox J M. Lysine-mannitol-glycerol agar, a medium for the isolation of Salmonella spp., including S. typhi and atypical strains ［J］. Appl Environ Microbiol, 1993, 59(8): 2602-2606.
|
|
Sun W, Zhao H, Hu S, et al. Verification And Analysis of Salmonella Test Ability in A Chocolate ［J］. Modern Food, 2018, (23): 116-120.
|
| ［12］ |
Peng E C, Wang J J. A study of production of radioactive environmental reference materials used for proficiency testing program in Taiwan ［J］. Appl Radiat lsot, 2013, 81: 14-20.
|
| ［13］ |
柯璐, 林杰, 戴晓丽, 等.肠炎沙门氏菌活菌标准物质的研制［J］.食品研究与开发,2015, 36(5): 111-115.
|
| ［14］ |
Vallejo C V, Tere C P, Calderon M N, et al. Development of a genomic DNA reference material for Salmonella enteritidis detection using polymerase chain reaction ［J］. Mol Cell Probes, 2021, 55: 101690.
|
|
Luo H P, Tang S, Chen Y W, et al. Preparation of Quality Control Samples of Salmonella And Their Application in The Proficiency Test ［J］. Journal of Food Safety and Quality, 2016, 7(4): 1473-1478.
|
| ［18］ |
GB 4789.2—2013 食品安全国家标准 食品微生物学检验 培养基和试剂的质量要求 ［S］. 2013.
|
|
Sui Z W, Wang B, Liu S Y, et al. Study on Measurement Method of Bacillus subtilis var. niger Spore ［J］. Acta Metrologica Sinica, 2020, 41(9): 1171-1176.
|
|
Wang B, Sui Z W, Liu S Y, et al. Rapid and Quantitative Detection of Viable Staphylococcus aureus in Milk Powder Based on Flow Cytometry Technology［J］. Acta Metrologica Sinica, 2021, 42(2): 250-258.
|
|
Li L Q, Wang Z Q, Liu S Y, et al. Study on Measurement Method of Rhodobacter Sphaeroides Based on Plate Counting［J］. Acta Metrologica Sinica, 2023, 44(3): 350-355.
|
|
Xu Y X, Gao W M, Sun G Y, et al. Experimental Observation on The Effect of Two Kinds of Salmonella Enrichment Solution in Food ［J］. Journal of Practical Medical Techniques, 2017, 24(11): 1184-1186.
|
| ［24］ |
张亓, 李苨.不同增菌和培养条件下沙门氏菌检出效果的比较［J］.工业微生物,2019, 49(1): 50-54.
|
|
Ke L, Lin J, Dai X L, et al. The Reference Materials of Preparation about Salmonella Enteritidis ［J］. Food Research and Development, 2015, 36(5): 111-115.
|
|
Zhang Q, Li N. Comparison of The Detection Effect of Salmonella Under Different Increasing And Culture Conditions ［J］. Industrial Microbiology, 2019, 49(1): 50-54.
|
| ［26］ |
杜发祥, 许菊霞, 杜雪琴, 等.食品中沙门氏菌能力验证［J］.食品安全导刊,2020, 4 (24): 120-121.
|
| ［16］ |
JJF 1343—2012 标准物质定值的通用原则及统计学原理 ［S］. 2012.
|
| ［3］ |
Besser J M. Salmonella epidemiology: a whirlwind of change ［J］. Food Microbiol, 2018, 71: 55-59.
|
| ［11］ |
JJF 1005—2016 标准物质通用术语和定义 ［S］. 2016.
|
| ［19］ |
GB 4789.2—2016 食品安全国家标准 食品微生物学检验 菌落总数测定 ［S］. 2016.
|
| ［23］ |
许云萧, 高卫明, 孙鸽云, 等.食品中沙门菌两种增菌液增菌效果的实验观察研究［J］.实用医技杂志,2017, 24(11): 1184-1186.
|
| ［15］ |
骆海朋, 唐颂, 陈怡文, 等.沙门氏菌能力验证样品的研制及其应用［J］.食品安全质量检测学报,2016, 7(4): 1473-1478.
|
| ［21］ |
王斌,隋志伟,刘思渊, 等. 基于流式分析技术的奶粉中金黄色葡萄球菌活菌快速定量检测方法研究［J］. 计量学报, 2021, 42(2): 250-258.
|
| ［22］ |
李龙泉,王梓权,刘思渊, 等. 基于平板计数的类球红细菌测量方法研究［J］. 计量学报, 2023, 44(3): 350-355.
|
| ［1］ |
Sahu B, Singh S D, Behera B K, et al. Rapid detection of Salmonella contamination in seafoods using multiplex PCR ［J］. Brazilian Journal of Microbiology, 2019, 50(3): 807-816.
|
| ［6］ |
McDonough P L, Shin S J, Lein D H. Diagnostic and public health dilemma of lactose-fermenting Salmonella enterica serotype typhimurium in cattle in the Northeastern United States ［J］. J Clin Microbiol, 2000, 38(3): 1221-1226.
|
| ［8］ |
Mouro J, Rebelo A, Ribeiro S, et al. Atypical non-H2S-producing monophasic Salmonella Typhimurium ST3478 strains from chicken meat at processing stage are adapted to diverse stresses ［J］. Pathogens, 2020, 9(9): 701.
|
|
Chai H Y, Qiao L, Chen L. Verification And Analysis Detection Ability of Salmonella in Milk Powder ［J］. Capital Journal of Public Health, 2020, 14(1): 50-53.
|
| ［10］ |
孙葳, 赵虹, 胡嵩, 等.一次巧克力中沙门氏菌检验能力验证结果与分析［J］.现代食品,2018(23): 116-120.
|
| ［17］ |
CNAS-CL03-A001-2019 能力验证提供者认可准则在微生物领域的应用说明 ［S］. 2019.
|
| ［4］ |
GB 4789.4—2016 食品安全国家标准 食品微生物学检验沙门氏菌检验［S］. 2016.
|
| ［9］ |
柴洪艳, 乔璐, 陈亮.奶粉中沙门氏菌检测能力验证结果与分析［J］.首都公共卫生,2020,14(1): 50-53.
|
| ［20］ |
隋志伟, 王斌, 刘思渊, 等. 枯草芽孢杆菌黑色变种芽孢测量方法研究［J］. 计量学报, 2020, 41(9): 1171-1176.
|
| ［25］ |
朱梦, 杨穗珊, 林宝英.沙门氏菌检测能力验证结果分析［J］.现代食品,2018, 4 (17): 145-148.
|
|
Du F X, Xu J X, Du X Q, et al. Proficiency Testing of Salmonella in food ［J］. China Food Safety Magazine, 2020, 4(24): 120-121.
|
|
Zhu M, Yang S S, Lin B Y, et al. Analysis of Verification Results of Salmonella Detection Ability ［J］. Modern Food, 2018, 4(17): 145-148.
|
|
|
|
2023, Vol. 44 
