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| Preparation of Cucumber Green Mottle Mosaic Virus in Seed Powder Matrix Reference Material |
| YIN Xin-ying,XU Yi-ling,LIU Yi-luan,ZHANG Yi,LU Zhong-ting,YAN Shuang,CAO Ji-juan |
| Key Laboratory of Biotechnology and Bioresources Utilization of Ministry of Education, College of Life Sciences,Dalian Minzu University, Dalian, Liaoning 116600, China |
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Abstract Seed powder matrix reference materials for cucumber green mottle mosaic virus were developed in this study to resolve the problem of lack of qualitative standard sample. Seed batches of Cucurbita pepo L. naturally infected with CGMMV were used as raw materials. After the specificity identification and confirmation, CGMMV reference materials of Cucurbita pepo L. seed powder matrix were prepared, which has been confirmed by double antibody sandwich enzyme-linked immunosorbent (DAS-ELISA) assay and real-time fluorescence quantitative RT-PCR (RT-qPCR) method.The results demonstrated that the reference materials were stable. After the samples were certified by using collaborative characterization program with eight sophisticated participating laboratories, Shapiro Wilk test showed that the standard sample conforms to Normal distribution.Seed power matrix reference materials were certified contained CGMMV by Grubbs method. Based on above results, the matrix reference materials in accordance with the requirements of the national reference material, it can be used for laboratory quality control, capability verification, testing method verification, and other activities.
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Received: 17 November 2022
Published: 25 June 2023
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| Fund:National Key R&D Program of China |
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2023, Vol. 44 
