RNA内参在转录组测序质量控制中的应用

doi:10.3969/j.issn.1000-1158.2023.05.22

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Abstract Eukaryotic transcriptome is diverse and complex. RNA sequencing (RNA-seq) can be used to provide a global profile of the transcriptome, find biomarkers associated with dieases and support the diagnosis and treatment of complex diseases. The process of RNA-seq includes sample preparation, library preparation, sequencing and analysis. The technical errors that are introduced during all the workflow influence the accuracy of RNA-seq. These errors can be understood and mitigated through the use of RNA spike-in, which can determine quality control for RNA-seq and improve the reliability of RNA-seq data. Therefore, RNA spike-in and its applications are introduced, including normalization between samples, gene expression measurements, detection and quantification of diverse transcripts, which provides powerful reference for improving the quality of RNA-seq by using RNA spike-in effectively.

Key words： metrology RNA sequencing RNA spike-in reference material quality control

Received: 03 January 2023 Published: 18 May 2023

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TB99

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	Articles by authors
	ZHANG Yu
	WANG Xia
	ZHANG Yong-zhuo
	YANG Jia-yi
	NIU Chun-yan
	ZHAO Yang
	DONG Lian-hua

Cite this article:

ZHANG Yu,WANG Xia,ZHANG Yong-zhuo, et al. Applications of RNA Spike-in Quality Control for RNA Sequencing[J]. Acta Metrologica Sinica, 2023, 44(5): 818-825.

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http://jlxb.china-csm.org:81/Jwk_jlxb/EN/10.3969/j.issn.1000-1158.2023.05.22 OR http://jlxb.china-csm.org:81/Jwk_jlxb/EN/Y2023/V44/I5/818

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