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| The Research of Quantification for HPV16 and HPV18(E6/E7) RNA Pseudovirus Nucleic Acid Reference Materials |
| LI Hui-jie1,CHEN Gui-fang3,GAO Yun-hua2,YANG Jia-yi2,DONG Lian-hua2 |
1. Shenzhen Institute for Technology Innovation, National Institute of Metrology, Shenzhen, Guangdong 518107,China
2. Center for Advanced Measurement Science, National Institute of Metrology, Beijing 100029,China
3. College of Chemistry Engineering, Shenyang University of Chemistry Technology,Shenyang, Liaoning 110142, China |
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Abstract Pseudoviruses containing E6/E7 RNA sequences of HPV16 and HPV18 were selected as reference materials. One-step reverse transcription digital PCR method was used to quantify content of E6/E7 RNA. HPV16 and HPV18 (E6/E7) RNA pseudoviruses were prepared and certificated (NIM-RM5231 and NIM-RM5232), which showed good uniformity and stability. The reference values with expanded uncertainty of the two reference materials were: (9.7±1.8)×102copies/μL, (5.9±1.1)×102copies/μL. The values were verified by multiple laboratories, and the relative standard deviations of the determination results were all less than 10%. The reference materials can provide reference for the quality control of the related detection of high-risk HPV E6/E7 RNA.
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Received: 18 July 2022
Published: 08 March 2023
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2023, Vol. 44 
