Abstract The research of bioaerosol relies on a stable and reliable atomization process. The performances of different aerosol generators are evaluated from the aspects of particle concentration, particle size distribution, bacterial culturability and cell membrane damage, and the influence of inlet flow is discussed.The results show that the mechanical stress in the process of aerosol generation seriously damages the integrity of the cell membrane, which reduces the cultivable rate of bacteria by more than 90%, and with the increase of air flow, the proportion of cell debris increases, and the cell membrane damage becomes more serious. Since the research of bioaerosol not only requires high concentrations of microorganisms, but also ensures the integrity and cultivability of cells, the above study will provide a basis for the performance evaluation and selection of bioaerosol generators.
TIAN Ying,ZHANG Guo-cheng,LI Jing-jing, et al. Evaluation of the Influence of Bioaerosol Atomizers on the Activity of E.coli[J]. Acta Metrologica Sinica, 2022, 43(5): 696-700.
［1］潘一廷, 张国城, 杨振琪,等. 生物气溶胶监测仪校准方法的研究［J］. 中国测试, 2020, 46(10):18-22.
Pan Y T, Zhang G C, Yang Z Q, et al. Research on calibration method of bioaerosol monitor instrument［J］. China Measurement and Test, 2020, 46(10):18-22.
［2］田莹,张国城,吴丹,等. 生物气溶胶监测仪生物计数评价装置的搭建和方法研究［J］. 计量学报,2021, 42(12): 1686-1691.
Tian Y, Zhang G C, Wu D, et al. Device Construction and Method Research of Biological Counting Evaluation of Bioaerosol Detectors［J］. Acta Metrologica Sinica, 2021, 42(12): 1686-1691.
［3］刘佳琪,张国城,赵晓宁,等. 进气流量对PM2.5切割器捕集效率的影响分析［J］. 计量学报,2021,42(4): 532-536.
Liu J Q, Zhang G C, Zhao X N, et al. The influence of air inlet flow rate on the capture efficiency of PM2.5 Cutter［J］. Acta Metrologica Sinica, 2021,42(4): 532-536.
［4］孙帅杰,齐天缘,肖骥,等. 高准确度气溶胶静电计的研制及校准［J］. 计量科学与技术,2021(2):54-58.
Sun S J, Qi T Y, Xiao J, et al. Development and Calibration of High Accuracy Aerosol Electrometer［J］. Metrology Science and Technology, 2021(2): 54-58.
［5］Xu Z Q, Wu Y, Shen F X, et al. Bioaerosol Science, Technology, and Engineering: Past, Present, and Future［J］. Aerosol Science and Technology, 2011, 45(11):1337-1349.
［6］Zhen H J, Han T, Fennell E D, et al. A systematic comparison of four bioaerosol generators: Affect on culturability and cell membrane integrity when aerosolizing Escherichia coli bacteria［J］. Journal of Aerosol Science, 2014, 70: 67-79.
［7］Ulevicius V, Willeke K, Grinshpun S A, et al. Aerosolization of particles from a bubbling liquid: characteristics and generator development［J］. Aerosol Science and Technology, 1997, 26: 175-190.
［8］Ganan-Calvo A M, Barrero A. A novel pneumatic technique to generate steady capillary microjets［J］. Journal of Aerosol Science, 1999, 30: 117-125.
［9］Griffiths W D, DeCosemo G A L. The assessment of bioaerosols: a critical review［J］. Journal of Aerosol Science, 1994, 25: 1425-1458.
［10］Mainelis G, Berry D, An H R, et al. Design and performance of a single-pass bubbling bioaerosol generator［J］. Atmospheric Environment, 2005, 39: 3521-3533.
［11］Simon X, Duquenne P, Koehler V, et al. Aerosolisation of Escherichia coli and associated endotoxin using an improved bubbling bioaerosol generator［J］. Journal of Aerosol Science, 2011, 42: 517-531.
［12］Chen S K, Vesley D, Brosseau L M, et al. Evaluation of single-use masks and respirators for protection of health care workers against mycobacterial aerosols［J］. American Journal of Infection Control, 1994, 22(2): 65-74.
［13］Alsved M, Bourouiba L, Duchaine C, et al. Natural sources and experimental generation of bioaerosols: Challenges and perspectives［J］. Aerosol Science and Technology, 2020,54(5): 547-571.
［14］Haddrell A E, Thomas R J. Aerobiology: Experimental considerations, observations, and future tools［J］. Applied and Environmental Microbiology, 2017, 83(17):1-17.
［15］Astudillo A, Leung S S Y, Kutter E, et al. Nebulization effects on structural stability of bacteriophage PEV 44［J］. European Journal of Pharmaceutics and Biopharmaceutics, 2018, 125: 124-130.
［16］Zhen H J, Han T, Fennell D E, et al. Release of free DNA by membrane-impaired bacterial aerosols due to aerosolization and air sampling［J］. Applied and Environmental Microbiology, 2013, 79: 7780-7789.
［17］Peters T M, Leith D. Concentration measurement and counting efficiency of the aerodynamic particle sizer 3321［J］. Journal of Aerosol Science, 2003, 34: 627-634.
［18］He Q S, Yao M S. Integration of high volume portable aerosol-to-hydrosol sampling and qPCR in monitoring bioaerosols［J］. Journal of environmental monitoring, 2011, 13(3): 706-12.
［19］Stein S W, Myrdal P B, Gabrio B J, et al. Evaluation of a new aerodynamic particle sizer spectrometer for size distribution measurements of solution metered dose inhalers［J］. Journal of Aerosol Medicine, 2003, 16: 107-119.
［20］Thomas R J, Webber D, Hopkins R, et al. The cell membrane as a major site of damage during aerosolization of Escherichia coli［J］. Applied and Environmental Microbiology, 2011, 77: 920-925.
［21］Stewart S L, Grinshpun S A, Willeke K, et al. Effect of impact stress on microbial recovery on an agar surface［J］. Applied and Environmental Microbiology, 1995, 61:1232-1239.
2022, Vol. 43 
