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| Development of Reference Material for White Blood Cell Count in Urine Sediment |
| LIU Ying-ying1,ZHANG Ling1,YANG Qiao-si2 |
1. National Institute of Metrology, Beijing, 100029, China
2. Dirui Industrial CO. LTD., Changchun, Jilin 130000, China |
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Abstract After the cell morphology of the fixed human white blood cells was investigated by scanning electron microscopy, the reference material candidate of white blood cell count in urine sediment was prepared. High throughput microscopic imaging technology and flow cytometry were used to jointly determine the value of reference material candidates; the homogeneity and stability were investigated by variance analysis and regression curve; the interoperability of matrix effect was verified with 20 clinical samples, and the uncertainty of reference material was evaluated. The concentration of the standard substance for white blood cell count in urine sediment is (516.0±50.5 )cells/μL(k=2), which has good uniformity and stability. It can be stored for 12 months at 4 ℃, and has good interoperability with clinical samples. The reference material can be used for the periodic calibration and quality control of white blood cell count by the automatic urine sediment analyzer in clinical detection, as well as the confirmation and validation of analytical methods of relevant tests.
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Received: 16 April 2020
Published: 23 March 2022
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| Fund:National Key R&D Program of China |
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2022, Vol. 43 
