基于时域分光法的微小容量测量方法研究

doi:10.3969/j.issn.1000-1158.2021.09.10

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Abstract In order to adapt to the current situation of the application of micro capacity and realize the on-line measurement of micro capacity, a micro capacity detection method based on time-domain spectrophotometry is studied. Based on the relationship between the absorbance of solution and the concentration of solution, this method reduces the influence of the processing error of the experimental consumables through the dual wavelength measurement. Based on the time-domain spectroscopic mode, the grating spectrophotometer is used to separate the monochromatic light from the polychromatic light source in the time domain, and then the detector detects the change of the absorbance of solution. Finally, the volume of solution is calculated. At the same time, the actual solution with different concentration can be prepared many times of measurement, to improved the utilization rate of experimental consumables. At 100μL and 50μL, the measurement value of liquid capacity is 99.67μL and 50.11μL, respectively, and the repeatability is 0.35% and 1.22%. Compared with the traditional gravity method, the same conclusion is obtained, which proves the feasibility of the micro capacity measurement method based on time-domain spectrophotometry.

Key words： metrology micro capacity time domain light splitting dual wavelength measurement

Received: 30 December 2019 Published: 24 September 2021

PACS:

TB938.3

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	Articles by authors
	LIU Xin-xing
	ZHANG Dian-long
	ZHANG Jing-yue
	SUN Bin
	TONG-Lin
	ZHAO Yu-xiao
	ZHONG Jia-dong

Cite this article:

LIU Xin-xing,ZHANG Dian-long,ZHANG Jing-yue, et al. Research on the Method of Small Capacity Measurement Based on Time Domain Spectrophotometry[J]. Acta Metrologica Sinica, 2021, 42(9): 1182-1186.

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http://jlxb.china-csm.org:81/Jwk_jlxb/EN/10.3969/j.issn.1000-1158.2021.09.10 OR http://jlxb.china-csm.org:81/Jwk_jlxb/EN/Y2021/V42/I9/1182

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