基于流式分析技术的奶粉中金黄色葡萄球菌活菌快速定量检测方法研究

doi:10.3969/j.issn.1000-1158.2021.02.19

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Abstract A flow cytometry (FCM)-based method for rapid and quantitative detection of viable Staphylococcus aureus in milk powder was developed. Staphylococcus aureus was labelled with Fluorescein isothiocyanate (FITC) by reacting with the anti-Staphylococcus aureus polyclonal antibody (FITC), then dead and viable bacteria were distinguished by membrane-impermeable dyes Propidium Iodid (PI). The FCM-based method was established and applied to the quantitative detection for Staphylococcus aureus in milk powder samples. The results showed that the FCM-based method exhibited high specificity towards Staphylococcus aureus. When the concentration of Staphylococcus aureus in artificially contaminated milk powder samples ranged from 2.72×10³~2.80×10⁸CFU/mL, the FCM-based method and plate-based method yielded the same result, and the detection limit of the method is 2.72×10³CFU/mL, and total time is 35min. The method is rapid and efficient, so it has a great application prospect in rapid screening and monitoring of foodborne pathogens.

Key words： metrology viable Staphylococcus aureus milk powder flow cytometry rapid detection

Received: 22 February 2019 Published: 18 February 2021

PACS:

TB99

Fund:National Key R&D Program of China;National Key R&D Program of China

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	Articles by authors
	WANG Bin
	SUI Zhi-wei
	LIU Si-yuan
	WANG Jing
	FU Bo-qiang
	WANG Yi

Cite this article:

WANG Bin,SUI Zhi-wei,LIU Si-yuan, et al. Rapid and Quantitative Detection of Viable Staphylococcus aureus in Milk Powder Based on Flow Cytometry Technology[J]. Acta Metrologica Sinica, 2021, 42(2): 250-258.

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http://jlxb.china-csm.org:81/Jwk_jlxb/EN/10.3969/j.issn.1000-1158.2021.02.19 OR http://jlxb.china-csm.org:81/Jwk_jlxb/EN/Y2021/V42/I2/250

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