|
|
|
| Research on Transgenic Cotton Mon15985 Plasmid Reference Material and Uncertainty Analysis |
| ZHANG Ling1,SHENG Ling-hui1,WANG Jing1,LIU Fang-fang2,LI Liang1,MI Wei1,DONG Lian-hua1 |
1. National Institute of Metrology, Beijing 100013, China;
2. Beijing University of Chemical Technology, Beijing 100029, China |
|
|
|
|
Abstract Method validation is researched on transgenic cotton Mon15985 quantitative realtime PCRA multilaboratory validation experiment is carriedThrough statistical analysis, the reference value of Mon15985 plasmid reference material is 1.02, with expanded uncertainty of 0.04(k=2)?Comparing plasmid molecules and transgenic cotton Mon15985 genomic DNA, both slope and linear correlation of the expansion curve are replaceableTherefore, the transgenic cotton Mon15985 plasmid reference material can be used as analytical testing standards in relevant transgene detection experiments.
|
|
|
|
|
|
［1］ISO Guide 35:2000,Certification of Reference Materials——General and Statistical Principles［S］.
［2］董莲华,李亮,王晶,等.转基因玉米pUC57-BT11质粒标准分子的构建与适用性研究［J］.计量学报,2011,32(6):570-574.
［3］董莲华,隋志伟,于笑波,等.转基因玉米NK603基体标准物质协同定值实验［J］.计量学报,2012,33(1):81-86.
［4］张玲,盛灵慧,王晶,等.转基因大豆Mon89788 基体标准物质协调实验研究［J］.计量学报,2011,32(6A):106-109.
［5］Randhawa G J, Chhabra R, Singh M. Decaplex and real-time PCR based detection of MON531 and MON15985 Bt cotton events［J］.J Agric Food Chem,2010, 58(18):9875-81.
［6］Kamle S,Kumar A,Bhatnagar R K. Development of multiplex and construct specific PCR assay for detection of cry2Ab transgene in genetically modified crops and product［J］.GM Crops,2011,2(1):74-81.
［7］杨晓杰,刘传亮,张朝军,等.不同转化方法获得的转基因棉花外源基因拷贝数分析［J］.农业生物技术学报,2011,19(2):221-229.
［8］全浩,韩永志．标准物质及其应用技术［M］．2版．北京:中国标准出版社,2003． |
|
|
|
2013, Vol. 34 
