聚合酶链式反应分析仪温度校准系统

doi:10.3969/j.issn.1000-1158.2018.05.11

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Abstract In order to calibrate the temperature of PCR instrument, a method was introduced for thermistor encapsulation, and a temperature calibration system for PCR instrument based on multi-thermistor sensors was developed, also the verification experiment for the system was accomplished. The experimental results demonstrated that the uniformity and the absolute value of indication error of the calibration system developed was below 0.15℃ and 0.06℃ respectively. While the extended uncertainty for indication error was less than 0.07℃ (k=2). In addition, a PCR instrument was calibrated using the developed system, and the parameters, such as indication error, uniformity, heat rate and cool rate, were all obtained.

Key words： metrology temperature calibration polymerase chain reaction thermistor encapsulation

Received: 10 May 2018 Published: 05 September 2018

PACS:

TB942

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	Articles by authors
	YU Song-lin
	WANG Zhe
	SHEN Wen-jie
	ZHANG Tao
	JIANG Jing
	TIAN Yun

Cite this article:

YU Song-lin,WANG Zhe,SHEN Wen-jie, et al. Temperature Calibration System for PCR Instrument[J]. Acta Metrologica Sinica, 2018, 39(5): 659-662.

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http://jlxb.china-csm.org:81/Jwk_jlxb/EN/10.3969/j.issn.1000-1158.2018.05.11 OR http://jlxb.china-csm.org:81/Jwk_jlxb/EN/Y2018/V39/I5/659

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