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| Study on Measurement Method of Serratia Marcescens |
| SUI Zhi-wei1,LIU Xiao-xia1,2,WANG Jing1,FU Bo-qiang1,XUE Lei1,LIU Si-zhang1,3,HAO Lin2 |
1. National Institute of Metrology, Beijing 100029, China
2. College of Food Science and Engineering, Shanxi Agricultural University, Taigu, Shanxi 030800, China
3. College of Life Science, Jilin Agricultural University, Changchun, Jilin 130118, China |
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Abstract Serratia marcescens was traditionally used as microbiology indicator of biological protection evaluation. Nutrient agar tablet coating method for Serratia marcescens were validated, collaborative experimental verified, and uncertainty analyzed. The results showed that nutrient agar tablet coating method had a good repeatability and reproducibility, the interassay relative standard deviations was 8.93%, and external laboratory relative standard deviations was 14.95%. The statistic test showed that the relative expanded uncertainty of nutrient agar tablet coating method was 12.60% (k=2), which could be used for quantitative measurement of Serratia marcescens.
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Received: 11 November 2016
Published: 06 July 2018
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［1］袁敏, 卢洪洲.科学防控埃博拉病毒病［J］. 中华传染病杂志, 2014, 32(9):515-516.
［2］张云辉, 王姝, 陈玉琪,等. 埃博拉出血热研究现状及2014年疫情进展［J］. 传染病信息, 2014, 27(4):3-8.
［3］祁国明. 病原微生物实验室生物安全［M］. 北京: 人民卫生出版社, 2005:1-3.
［4］杨瑞馥, 王松俊. 生物威胁与核查［M］. 北京: 军事医学科学出版社, 2003: 182-193.
［5］沈伟, 何静芳, 苏怡,等.医用防护服与防护口罩阻隔性能研究［J］.中国消毒学杂志, 2005, 22(4):386-390.
［6］鹿建春, 孙振海, 李劲松,等. 生物防护口罩的研制及其防护效果评价［J］.中国消毒学杂志, 2004, 21(3):189-192.
［7］赵月峨, 鹿建春, 温占波,等. 一种新型高效生物防护口罩的防护性能测试［J］.中华预防医学杂志, 2007, 8(1):27-29.
［8］赵月峨, 鹿建春, 温占波,等. 一种达到面具防护水平的生物防护口罩［J］. 生物防护防疫装备, 2006, 27(12):34-36.
［9］沈恒根, 杨磊, 刘刚,等. 新型医用防护口罩过滤环境气溶胶的性能［J］. 第二军医大学学报, 2003, 24(6):629-631.
［10］朱孟府, 龚承元, 苏建勇,等. 平面型荷电防病毒口罩的结构及性能评价［J］. 生物防护防疫装备, 2005, 26(4):29-30.
［11］毕建军. 生物安全设施和设备生物学验证方法的研究及应用［D］. 北京:中国人民解放军军事医学科学院, 2006.
［12］王洁, 温占波, 赵建军,等. 个人防护装备高效过滤罐微生物气溶胶防护效果的检测及评价［J］. 中国医学装备, 2007, 4(3):33-36.
［13］毕建军, 温占波, 赵建军,等.用粘质沙雷菌试验评价Ⅱ级生物安全柜的研究［J］. 中国消毒学杂志, 2007, 24(1):4-8.
［14］GB/T 6379.2—2004 测量方法与结果的准确度（正确度与精密度）第2部分:确定标准测量方法重复性与再现性的基本方法［S］.
［15］ISO Guide 35:2006. Reference materials—General and statistical principles for certification［S］.
［16］JJF 1343—2012 标准物质定值的通用原则及统计学原理［S］.
［17］JJG 1006—94 一级标准物质［S］.
［18］Parenteral Drug Association. Evaluation, Validation and Implementation of New as Microbiological Testing Methods: Technical Report No.33［R］. Iowa City:PDA, 2000.
［19］全浩, 韩永志. 标准物质及其应用技术［M］. 2版. 北京: 中国标准出版社, 2003. |
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2018, Vol. 39 
